Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/79166
Type: Artigo de periódico
Title: DAMAGE TO RAT-LIVER MITOCHONDRIA PROMOTED BY DELTA-AMINOLEVULINIC ACID-GENERATED REACTIVE OXYGEN SPECIES - CONNECTIONS WITH ACUTE INTERMITTENT PORPHYRIA AND LEAD-POISONING
Author: HERMESLIMA, M
VALLE, VGR
VERCESI, AE
BECHARA, EJH
Abstract: delta-Aminolevulinic acid is a heme precursor accumulated in acute intermittent porphyria and lead-poisoning, which supposedly triggers the typical clinical expression associated with these diseases. Considering that: (i) erythrocyte anti-oxidant enzymes are abnormally high in patients with both disorders and (ii) delta-aminolevulinic acid autoxidation generates reactive oxygen species, a possible contribution of reactive oxygen species in the pathophysiology of these disorders is explored here. Evidence is provided that delta-aminolevulinic acid (2-15 mM) induces damage to isolated rat liver mitochondria. Addition of delta-aminolevulinic acid disrupts the mitochondrial membrane potential, promotes Ca2+ release from the intramitochondrial matrix and releases the state-4 respiration, thus enhancing the permeability of the membrane to H+. The lesion was abolished by catalase, superoxide dismutase (both enzymes inhibit delta-aminolevulinic acid autoxidation) and ortho-phenanthroline, but not by mannitol; added H2O2 induces damage poorly. These results suggest the involvement of deleterious reactive oxygen species formed at particular mitochondrial sites from transition metal ions and delta-aminolevulinic acid-generated peroxide and/or superoxide species. These observations might be compatible with previous work showing hepatic mitochondrial damage in liver biopsy samples of acute intermittent porphyria patients.
Subject: DELTA-AMINOLEVULINIC ACID
PORPHYRIA, ACUTE INTERMITTENT
LEAD POISONING
REACTIVE OXYGEN SPECIES
OXYGEN
MITOCHONDRIAL DAMAGE
(RAT LIVER MITOCHONDRIA)
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/S0005-2728(05)80072-6
Date Issue: 1991
Appears in Collections:Unicamp - Artigos e Outros Documentos

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