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Type: Artigo de periódico
Title: Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A
Author: Ferreira, HL
Spilki, FR
dos Santos, MMAB
de Almeida, RS
Arns, CW
Abstract: Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F) gene and nucleocapsid (N) gene were compared with an established test for the attachment (G) gene. All the RT-PCR tested assays were able to detect the AMPV/A. The lower detection limits were observed using the N-, F-based RRT-PCR and F-based conventional RT-PCR (10(0.3) to 10(1) TCID(50) mL(-1)). The present study suggests that the conventional F-based RT-PCR presented similar detection limit when compared to N-and F-based RRT-PCR and they can be successfully used for AMPV/A detection.
Subject: avian metapneumovirus
G, F, N genes
real time RT-PCR
Country: Brasil
Editor: Univ Federal Santa Maria
Citation: Ciencia Rural. Univ Federal Santa Maria, v. 39, n. 5, n. 1445, n. 1451, 2009.
Rights: aberto
Identifier DOI: 10.1590/S0103-84782009005000057
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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