Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/79143
Type: Artigo de periódico
Title: Comparative effect of Phoneutria nigriventer spider venom and capsaicin on the rat paw oedema
Author: Costa, SKP
Esquisatto, LCM
Camargo, E
Gambero, A
Brain, SD
De Nucci, G
Antunes, E
Abstract: Capsaicin, the pungent component of hot peppers, and the venom of the spider Phoneutria nigriventer are able to activate sensory nerves resulting in cutaneous neurogenic plasma extravasation. This study was undertaken to compare the ability of these substances to evoke oedema in the rat hind-paw and mechanisms underlying this effect. Subplantar injection of either Phoneutria nigriventer venom (PNV; 1-100 mug/paw) or capsaicin (10-200 mug/paw) caused a significant paw oedema that was potentiated by CGRP (10 pmol/paw). In rats treated neonatally with capsaicin to deplete neuropeptides. the paw oedema induced by either PNV (100 mug/paw) or capsaicin (100 mug/paw) was partially reduced (P <0.05). The tachykinin NKI receptor antagonist SR140333 (0.2 mu mol/kg; i.v.) prevented the paw oedema induced by the tachykinin NK1 receptor agonist GR73632 (30 pmol/paw) and partially reduced paw oedema induced by PNV or capsaicin. Treatment of rats with compound 48/80 (5 mg/kg; s.c. 3 days) or with both HI receptor antagonist (mepyramine; 1 nmol/paw) and 5-HT receptor antagonist (methysergide; I nmol/paw) significantly inhibited PNV- or capsaicin-induced paw oedema. The combined treatment with mepyramine and methysergide and SR140333 further reduced PNV- and capsaicin-induced paw oedema. The bradykinin B-2 receptor antagonist Hoe 140 affected neither PNV- nor capsaicin-induced responses. Our results suggest that PNV and capsaicin each induce paw oedema that is partially mediated by activation of sensory fibers culminating in the release of substance P as well as by activation of mast cells which in turn release amines such as histamine and 5-HT. (C) 2001 Elsevier Science Inc. All rights reserved.
Subject: Phoneutria nigriventer venom
sensory nerves
kinins
mast cells
neurogenic oedema
tachykinin NK1 receptor
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/S0024-3205(01)01234-6
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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