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Type: Artigo de periódico
Title: Comparative cytotoxicity of dimethylamide-crotonin in the promyelocytic leukemia cell line (HL60) and human peripheral blood mononuclear cells
Author: Anazetti, MC
Melo, PS
Duran, N
Haun, M
Abstract: Dehydrocrotonin (DHC) is a diterpene lactone obtained from Croton cajucara (Sacaca). Dimethylamide-crotonin (DCR), a DHC derivative, has a similar inhibitory effect on leukemic HL60 cells than its parent compound evaluated by different endpoints of cytotoxicity. No cytotoxicity or morphological alterations associated with apoptosis were detected in human peripheral blood mononuclear cells (PBMC) after treatment with up to 400 muM DCR in presence of phytohemaglutinin (5 mug/ml). Based on morphological changes and the pattern of DNA fragmentation, DHC and DCR were found to induce apoptosis and terminal differentiation (assessed by nitro blue tetrazolium reduction) in HL60 cells, but these compounds did not show any toxic effect in PBMC. Thus, DCR and DHC inhibit HL60 cell growth in vitro partly by inducing apoptosis and cell differentiation, but does not cause serious damage to immune cells according to our experimental conditions. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
Subject: apoptosis
cell differentiation
HL60 cells
human peripheral blood mononuclear
Country: Irlanda
Editor: Elsevier Sci Ireland Ltd
Citation: Toxicology. Elsevier Sci Ireland Ltd, v. 188, n. 41700, n. 261, n. 274, 2003.
Rights: fechado
Identifier DOI: 10.1016/S0300-483X(03)00089-1
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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