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Type: Artigo de periódico
Title: Colorimetric MTT assessment of antifungal activity of D0870 against fluconazole-resistant Candida albicans
Author: Yang, HC
Mikami, Y
Yazawa, K
Taguchi, H
Nishimura, K
Miyaji, M
Branchini, MLM
Aoki, FH
Yamamoto, K
Abstract: The in vitro antifungal activity of D0870 against eight isolates of fluconazole-resistant Candida albicans was compared with that of itraconazole, ketoconazole and miconazole. The colorimetric MTT [3-(4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H tetrazolium bromide] assay was used to assess the antifungal activities. The 50% minimum inhibitory concentration (MIC50) of D0870 was below 0.031 mu g ml(-1) for seven isolates and 0.25 mu g ml(-1) for one isolate. The activity of D0870 was superior to that of the other azoles. Ketoconazole was the most effective azole next to D0870. Therefore, the new bis-triazole, D0870, is expected to be promising for the therapy of fluconazole-resistant candidosis. The present data also confirmed that the MTT assay may be useful for evaluation of resistance and detection of resistant C. albicans.
Subject: Candida albicans
fluconazole resistance
azole antifungals
antifungal activity
MTT assay
Country: Alemanha
Editor: Blackwell Wissenschafts-verlag Gmbh
Citation: Mycoses. Blackwell Wissenschafts-verlag Gmbh, v. 41, n. 41984, n. 477, n. 480, 1998.
Rights: fechado
Date Issue: 1998
Appears in Collections:Unicamp - Artigos e Outros Documentos

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