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Type: Artigo de periódico
Title: Temperature and pH conditions for maximum activity of bromelain extracted from pineapple (Ananas Comosus L. Merril)
Author: Elias, MJ
Arcuri, IF
Tambourgi, EB
Abstract: Temperature and pH conditions for maximum activity of bromelain extracted from pineapple (Ananas Comosus L. Merril) Bromelain, the enzyme found in pineapple, hydrolyzes proteins' peptide bonds, has several applications in the food industry and in medicine. Temperature and pH conditions for highest activity with casein as substrate were investigated from the bromelain recovered from industrialized pineapple ('perola' variety) fruit residues. Extract was obtained by grinding fruit rind and its internal stem. The activity was expressed in mmol tyrosine L(-1) min.(-1) by absorbance at 280 nm of aromatic amino acids produced in casein hydrolysis. Assays were undertaken in duplicate for two enzyme/substrate with ratios: 1/25 and 1/125 (mass). Eight-pointed experimental design was undertaken with central value for pH at 7.0 and for temperature at 35 degrees C. Experimental results were processed by MINITAB 15 (Minitab Inc) software which provided model's equations and surface responses. Equations were mathematically processed by differential calculus producing graphs which exhibit the best activities according to temperatures. Loss of enzyme activity was reported with temperature increase at 1/25 ratio, due to a higher free enzyme, when compared to 1/125 ratio.
Subject: enzyme
thermal decomposition
sulphhydril protease
Country: Brasil
Editor: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao
Citation: Acta Scientiarum-technology. Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 33, n. 2, n. 191, n. 196, 2011.
Rights: aberto
Identifier DOI: 10.4025/actascitechnol.v33i2.7928
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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