Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/78759
Type: Artigo de periódico
Title: COAGGLUTINATION ASSAY FOR DETECTION OF ROTAVIRUSES
Author: PARREIRA, VRP
FERRAZ, MMG
GATTI, MSV
PESTANA, AFD
Abstract: Rotaviruses present in human and porcine faecal specimens were detected using a coagglutination test. A suspension of Staphylococcus aureus, protein A-positive, coated with group A rotavirus diluted antiserum agglutinated specifically faecal extracts known to be positive for rotavirus by the EIERA test. A total of 89 faecal samples absorbed with S. aureus were tested by the coagglutination test and compared with an enzyme immunoassay (EIERA). The mixture of staphyloccocal cells and diluted serum showed a stability up to 6 months when stored at 4-degrees-C. Statistical analysis of the results showed a close correlation between the two tests (C=0.91). It was concluded that the coagglutination test was a simple, rapid, cheap and sensitive assay for the detection of rotavirus in faecal specimens for routine work.
Subject: ROTAVIRUS
COAGGLUTINATION ASSAY
Country: Brasil
Editor: Soc Brasileira Microbiologia
Rights: aberto
Date Issue: 1992
Appears in Collections:Unicamp - Artigos e Outros Documentos

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