Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/78669
Type: Artigo de periódico
Title: THAPSIGARGIN CAUSES CA2+ RELEASE AND COLLAPSE OF THE MEMBRANE-POTENTIAL OF TRYPANOSOMA-BRUCEI MITOCHONDRIA INSITU AND OF ISOLATED RAT-LIVER MITOCHONDRIA
Author: VERCESI, AE
MORENO, SNJ
BERNARDES, CF
MEINICKE, AR
FERNANDES, EC
DOCAMPO, R
Abstract: Thapsigargin, previously reported to release Ca2+ from non-mitochondrial stores of different cell types, as well as nigericin, were found, when used at high concentrations, to release Ca2+ and collapse the membrane potential of Trypanosoma brucei bloodstream and procyclic trypomastigotes mitochondria in situ. At similarly high concentrations (>10 muM), thapsigargin was also found to release Ca2+ and collapse the membrane potential of isolated rat liver mitochondria. These results indicate that care should be taken when attributing the effects of thapsigargin in intact cells to the specific inhibition of the sarcoplasmic and endoplasmic reticulum Ca2+-ATPase family of calcium pumps. In addition, we have found no evidence for an increase in intracellular Ca2+ by release of the ion from intracellular stores by nigericin, measuring changes in cytosolic Ca2+ by dual wavelength spectrofluorometry in fura-2-loaded T. brucei bloodstream trypomastigotes or measuring Ca2+ transport in digitonin-permeabilized cells.
Editor: Amer Soc Biochemistry Molecular Biology Inc
Rights: fechado
Date Issue: 1993
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File Description SizeFormat 
WOSA1993KX81100031.pdf580.97 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.