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Type: Artigo de periódico
Title: Cementoblast gene expression is regulated by Porphyromonas gingivalis lipopolysaccharide partially via Toll-like receptor-4/MD-2
Author: Nociti, FH
Foster, BL
Barros, SP
Darveau, RP
Somerman, MJ
Abstract: Lipopolysaccharides are potent inflammatory mediators considered to contribute to destruction of periodontal tissues. Here, we hypothesized that Porphyromonas gingivalis lipopolysaccharide (P-LPS) treatment would regulate gene expression in murine cementoblasts through Toll-like receptor 4. Real-time (RT)-PCR and Northern blot analysis indicated that P-LPS decreased expression of transcripts for osteocalcin (OCN) and receptor activator of nuclear factor kappaB ligand (RANKL). In contrast, a dose-dependent up-regulation in mRNA levels for osteopontin (OPN) and osteoprotegerin (OPG) was observed. Similarly, ELISA demonstrated decreased RANKL and increased OPG levels. A monoclonal antibody specific for mouse TLR-4/MD-2 partially neutralized the P-LPS effect on cementoblasts. These results indicate that exposure of cementoblasts to P-LPS can alter cell function by regulating markers of osteoclastic activity (e.g., RANKL/OPG), thereby potentially affecting the inflammation-associated resorption of mineralized tissues.
Subject: lipopolysaccharides
Porphyromonas gingivalis
Country: EUA
Editor: Int Amer Assoc Dental Researchi A D R/a A D R
Citation: Journal Of Dental Research. Int Amer Assoc Dental Researchi A D R/a A D R, v. 83, n. 8, n. 602, n. 607, 2004.
Rights: fechado
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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