Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/78613
Type: Artigo de periódico
Title: Cellulitis lesions in broiler chickens are induced by Escherichia coli Vacuolating Factor (ECVF)
Author: Quel, NG
Aragao, AZB
Salvadori, MR
Farias, AS
Joazeiro, PP
Santos, LMB
Sa, LRM
Ferreira, AJP
Yano, T
Abstract: Escherichia coli Vacuolating Factor (ECVF) is a heat-labile, vacuolating cytotoxin produced by avian pathogenic E. coli (APEC) isolated from avian cellulitis lesions. In this report, we intend to demonstrate that purified ECVF induces the inflammatory process of cellulitis. Our group is the first to demonstrate the effect of ECVF in a histological analysis by in situ inoculation of broiler chickens with purified ECVF. The animals were inoculated with the APEC AC53 and with purified ECVF subcutaneously on their ventral surface (in the sternum region). The histological analysis showed different grades of an acute inflammatory response in the epidermis, dermis and panniculus. An increase in mRNA expression of the proinflammatory cytokine TNF-alpha was also demonstrated in the inflamed tissue. When ECVF was systemically administered, increased levels of TNF-alpha and IL-10 were observed in the serum. These results suggest that ECVF plays a key role in the inflammatory process associated with cellulitis that is mainly mediated by TNF-alpha. In addition, this inflammation can be downregulated by the anti-inflammatory cytokine IL-10. (C) 2012 Published by Elsevier B.V.
Subject: APEC
Avian cellulitis
Escherichia coli Vacuolating Factor (ECVF)
Purification
Cytokines
Inflammatory response
Dermatopathology
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.vetmic.2012.11.007
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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