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Type: Artigo de periódico
Title: Cell-to-cell contact dependence and junctional protein content are correlated with in vivo maturation of pancreatic beta cells
Author: Santos-Silva, JC
Carvalho, CPD
de Oliveira, RB
Boschero, AC
Collares-Buzato, CB
Abstract: In this study, we investigated the cellular distribution of junctional proteins and the dependence on cell-cell contacts of pancreatic beta cells during animal development. Fetus and newborn rat islets, which display a relatively poor insulin secretory response to glucose, present an immature morphology and cytoarchitecture when compared with young and adult islets that are responsive to glucose. At the perinatal stage, beta cells display a low junctional content of neural cell adhesion molecule (N-CAM), alpha-and beta-catenins, ZO-1, and F-actin, while a differential distribution of N-CAM and Pan-cadherin was seen in beta cells and nonbeta cells only from young and adult islets. In the absence of intercellular contacts, the glucose-stimulated insulin secretion was completely blocked in adult beta cells, but after reaggregation they partially re-established the secretory response to glucose. By contrast, neonatal beta cells were poorly responsive to sugar, regardless of whether they were arranged as intact islets or as isolated cells. Interestingly, after 10 days of culturing, neonatal beta cells, known to display increased junctional protein content in vitro, became responsive to glucose and concomitantly dependent on cell-cell contacts. Therefore, our data suggest that the developmental acquisition of an adult-like insulin secretory pattern is paralleled by a dependence on direct cell-cell interactions.
Subject: pancreatic beta cells
intercellular junctions
beta cell maturation
pancreas development
cell adhesion
junctional proteins
insulin secretion
Country: Canadá
Editor: Canadian Science Publishing, Nrc Research Press
Citation: Canadian Journal Of Physiology And Pharmacology. Canadian Science Publishing, Nrc Research Press, v. 90, n. 7, n. 837, n. 850, 2012.
Rights: fechado
Identifier DOI: 10.1139/Y2012-064
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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