Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/78549
Type: Artigo de periódico
Title: Tendency for oxidation of annelid hemoglobin at alkaline pH and dissociated states probed by redox titration
Author: Bispo, JAC
Landini, GF
Santos, JLR
Norberto, DR
Bonafe, CFS
Abstract: The redox titration of extracellular hemoglobin of Glossoscolex paulistus (Annelidea) was investigated in different pH conditions and after dissociation induced by pressure. Oxidation increased with increasing PH, as shown by the reduced amount of ferricyanide necessary for the oxidation of hemoglobin. This behavior was the opposite of that of vertebrate hemoglobins. The potential of half oxidation (E 1/2) changed from - 65.3 to + 146.8 mV when the pH increased from 4.50 to 8.75. The functional properties indicated a reduction in the log P-50 from 1.28 to 0.28 in this pH range. The dissociation at alkaline pH or induced by high pressure, confirmed by HPLC gel filtration, suggested that disassembly of the hemoglobin could be involved in the increased potential for oxidation. These results suggest that the high stability and prolonged lifetime common to invertebrate hemoglobins is related to their low tendency to oxidize at acidic pH, in contrast to vertebrate hemoglobins. (c) 2005 Elsevier Inc. All rights reserved.
Subject: annelid hemoglobin
extracellular hemoglobin
erythrocruorin
dissociated hemoglobin
Glossoscolex paulistus
hemoglobin oxidation
pressure-induced dissociation
redox potential titration
Country: EUA
Editor: Elsevier Science Inc
Rights: fechado
Identifier DOI: 10.1016/j.cdpc.2005.06.002
Date Issue: 2005
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File Description SizeFormat 
WOS000230985900013.pdf263.98 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.