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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleCathepsin K Is Present in Invasive Oral Tongue Squamous Cell Carcinoma In Vivo and In Vitropt_BR
dc.contributor.authorBitu, CCpt_BR
dc.contributor.authorKauppila, JHpt_BR
dc.contributor.authorBufalino, Apt_BR
dc.contributor.authorNurmenniemi, Spt_BR
dc.contributor.authorTeppo, Spt_BR
dc.contributor.authorKeinanen, Mpt_BR
dc.contributor.authorVilen, STpt_BR
dc.contributor.authorLehenkari, Ppt_BR
dc.contributor.authorNyberg, Ppt_BR
dc.contributor.authorColetta, RDpt_BR
dc.contributor.authorSalo, Tpt_BR
unicamp.authorBitu, Carolina C. Nurmenniemi, Sini Teppo, Susanna Vilen, Suvi-Tuuli Nyberg, Pia Salo, Tuula Univ Oulu, Dept Diagnost & Oral Med, Inst Dent, Oulu, Finlandpt_BR
unicamp.authorKauppila, Joonas H. Univ Oulu, Dept Pathol, Inst Diagnost, Oulu, Finlandpt_BR
unicamp.authorKauppila, Joonas H. Univ Oulu, Dept Surg, Inst Clin Med, Oulu, Finlandpt_BR
unicamp.authorVilen, Suvi-Tuuli Salo, Tuula Univ Helsinki, Biomedicum Helsinki Fac Med, Inst Dent, Dept Cell Biol Oral Dis, Helsinki, Finlandpt_BR
unicamp.authorKeinanen, Meeri Lehenkari, Petri Univ Oulu, Dept Anat & Cell Biol, Oulu, Finlandpt_BR
unicamp.authorKauppila, Joonas H. Lehenkari, Petri Salo, Tuula Oulu Univ Hosp, Oulu, Finlandpt_BR
unicamp.authorBufalino, Andreia Coletta, Ricardo D. Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Piracicaba, SP, Brazilpt_BR
dc.subject.wosTumor Microenvironmentpt_BR
dc.subject.wosSkin Fibroblastspt_BR
dc.description.abstractObjectives: Cathepsin K, a lysosomal cysteine protease, is expressed in the tumor microenvironment (TME) of skin carcinoma, but nothing is known about cathepsin K in oral tongue squamous cell carcinoma (OTSCC). Our aim was to describe the expression of cathepsin K in invasive OTSCC in vitro and in a series of clinical cancer specimens. Materials and Methods: OTSCC invasion in vitro was studied using invasive HSC-3 tongue carcinoma cells in 3D organotypic models. In total, 121 mobile tongue OTSCCs and 10 lymph node metastases were analyzed for cathepsin K expression. The association between cathepsin K expression and clinicopathological factors was evaluated. Results: Cysteine protease inhibitor E64 and cathepsin K silencing significantly (p < 0.0001) reduced HSC-3 cell invasion in the 3D models. Cathepsin K was expressed in a majority of carcinoma and metastatic cells, but the expression pattern in carcinoma cells did not correlate with clinical parameters. Instead, the weak expression of cathepsin K in the invasive TME front correlated with increased overall recurrence (p < 0.05), and in early-stage tumors this pattern predicted both cancer recurrence and cancer-specific mortality (p < 0.05 and p < 0.005, respectively). Conclusions: Cathepsin K is expressed in OTSCC tissue in both carcinoma and TME cells. Although the diminished activity and expression in aggressive tongue HSC-3 cells reduced 3D invasion in vitro, the amount of cathepsin K in carcinoma cells was not associated with the outcome of cancer patients. Instead, cathepsin K in the invasive TME front seems to have a protective role in the complex progression of tongue
dc.relation.ispartofPlos Onept_BR
dc.relation.ispartofabbreviationPLoS Onept_BR
dc.publisher.citySan Franciscopt_BR
dc.publisherPublic Library Sciencept_BR 7pt_BR
dc.identifier.citationPlos One. Public Library Science, v. 8, n. 8, 2013.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipEmil Aaltonen Foundationpt_BR
dc.description.sponsorshipCancer Foundation of Northern Ostrobotniapt_BR
dc.description.sponsorshipOulu University Research Foundationpt_BR
dc.description.sponsorshipGeorg C. and Mary Ehrnrooth Foundationpt_BR
dc.description.sponsorshipFinnish Medical Foundationpt_BR
dc.description.sponsorshipAcademy of Finlandpt_BR
dc.description.sponsorshipSigrid Juselius Foundationpt_BR
dc.description.sponsorshipKevo grantspt_BR
dc.description.provenanceMade available in DSpace on 2014-08-01T18:24:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:59:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
Appears in Collections:Unicamp - Artigos e Outros Documentos

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