Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/78353
Type: Artigo de periódico
Title: CALCIUM-DEPENDENT MITOCHONDRIAL OXIDATIVE DAMAGE PROMOTED BY 5-AMINOLEVULINIC ACID
Author: HERMESLIMA, M
CASTILHO, RF
VALLE, VGR
BECHARA, EJH
VERCESI, AE
Abstract: Swelling of isolated rat liver mitochondria is shown to be induced by metal-catalyzed 5-aminolevulinic acid (ALA) aerobic oxidation, a putative endogenous source of reactive oxygen species (ROS), at concentrations as low as 50-100 muM. In this concentration range, AI-A is estimated to occur in the liver of acute intermittent porphyria patients. Removal of Ca2+ (10 muM) from the suspension of isolated rat liver mitochondria by added EGTA abolishes both the ALA-induced transmembrane-potential collapse and mitochondrial swelling. Prevention of the ALA-induced swelling by addition of ruthenium red prior to mitochondrial energization by succinate demonstrates the deleterious involvement of internal Ca2+. Addition of MgCl2 at concentrations higher than 2.5 mM, prevents the ALA-induced mitochondrial swelling, transmembrane potential collapse and Ca2+ efflux. This indicates that Mg2+ protects against the mitochondrial damage promoted by ALA-generated ROS. The ALA-induced mitochondrial damage might be a key event in the liver mitochondrial damage of acute intermittent porphyria patients reported elsewhere.
Subject: 5-AMINOLEVULINIC ACID
PORPHYRIA
REACTIVE OXYGEN SPECIES
CALCIUM ION HOMEOSTASIS
CALCIUM ION
MAGNESIUM ION
MITOCHONDRIAL DAMAGE
(RAT LIVER MITOCHONDRION)
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/0925-4439(92)90069-Y
Date Issue: 1992
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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