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Type: Artigo de periódico
Title: Calcium imaging of muscle cells treated with snake myotoxins reveals toxin synergism and presence of acceptors
Author: Cintra-Francischinelli, M
Pizzo, P
Rodrigues-Simioni, L
Ponce-Soto, LA
Rossetto, O
Lomonte, B
Gutierrez, JM
Pozzan, T
Montecucco, C
Abstract: Snake myotoxins have a great impact on human health worldwide. Most of them adopt a phospholipase A2 fold and occur in two forms which often co-exist in the same venom: the Asp49 toxins hydrolyse phospholipids, whilst Lys49 toxins are enzymatically inactive. To gain insights into their mechanism of action, muscle cells were exposed to Bothrops myotoxins, and cytosolic Ca(2+) and cytotoxicity were measured. In both myoblasts and myotubes, the myotoxins induced a rapid and transient rise in cytosolic [Ca(2+)], derived from intracellular stores, followed, only in myotubes, by a large Ca(2+) influx and extensive cell death. Myoblast viability was unaffected. Notably, in myotubes Asp49 and Lys49 myotoxins acted synergistically to increase the plasma membrane Ca(2+) permeability, inducing cell death. Therefore, these myotoxins may bind to acceptor(s) coupled to intracellular Ca(2+) mobilization in both myoblasts and myotubes. However, in myotubes only, the toxins alter plasma membrane permeability, leading to death.
Subject: Snake myotoxins
calcium imaging
Country: Suíça
Editor: Birkhauser Verlag Ag
Citation: Cellular And Molecular Life Sciences. Birkhauser Verlag Ag, v. 66, n. 10, n. 1718, n. 1728, 2009.
Rights: fechado
Identifier DOI: 10.1007/s00018-009-9053-2
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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