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|Type:||Artigo de periódico|
|Title:||Ca2+ transport into an intracellular acidic compartment of Candida parapsilosis|
|Abstract:||In this report, we study Ca2+ transport in permeabilized Candida parapsilosis spheroplasts prepared by a new technique using lyticase, An intracellular non-mitochondrial Ca2+ uptake pathway, insensitive to orthovanadate and sensitive to the V-H+-ATPase inhibitor bafilomycin A(1), nigericin and carbonyl cyanide p-trifluoromethoxyphenylhydrazone was characterized, Acidification of the compartment in which Ca2+ accumulated was followed using the fluorescent dye acridine orange. Acidification was stimulated by the Ca2+ chelator EGTA and inhibited by Ca2+ These results, when added to the observation that Ca2+ induces alkalization of a cellular compartment, provide evidence for the presence of a Ca2+/nH(+) antiporter in the acid compartment membrane. Interestingly, like in acidocalcisomes of trypanosomatids, the antioxidant 3,5-dibutyl-4-hydroxytoluene inhibits the V-H+-ATPase, In addition, the antifungal agent ketoconazole promoted a fast alkalization of the acidic compartment, Ketoconazole effects mere dose-dependent and occurred in a concentration range close to that attained in the plasma of patients treated with this drug. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.|
|Editor:||Elsevier Science Bv|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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