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Type: Artigo de periódico
Title: The characterization of a thermostable and cambialistic superoxide dismutase from Thermus filiformis
Author: Mandelli, F
Cairo, JPLF
Citadini, APS
Buechli, F
Alvarez, TM
Oliveira, RJ
Leite, VBP
Leme, AFP
Mercadante, AZ
Squina, FM
Abstract: The superoxide dismutase (TfSOD) gene from the extremely thermophilic bacterium Thermus filiformis was cloned and expressed at high levels in mesophilic host. The purified enzyme displayed approximately 25 kDa band in the SDS-PAGE, which was further confirmed as TfSOD by mass spectrometry. The TfSOD was characterized as a cambialistic enzyme once it had enzymatic activity with either manganese or iron as cofactor. TfSOD showed thermostability at 65, 70 and 80 degrees C. The amount of enzyme required to inhibit 50% of pyrogallol autoxidation was 0 center dot 41, 0 center dot 56 and 13 center dot 73mg at 65, 70 and 80 degrees C, respectively. According to the circular dichroism (CD) spectra data, the secondary structure was progressively lost after increasing the temperature above 70 degrees C. The 3-dimensional model of TfSOD with the predicted cofactor binding corroborated with functional and CD analysis. Significance and Impact of the Study This manuscript describes the expression and characterization of a superoxide dismutase (SOD) from Thermus filiformis with thermophilic and cambialistic characteristics. The SODs are among the most potent antioxidants known in nature, and their stability and pharmacokinetics can vary widely in accordance to their biological source. Although the currently clinical research work has been focused on human and bovine SODs, alternative sources may become more biotechnological attractive in the near future. Our study brings new insights for the research field of antioxidant enzymes with potential application on pharmaceutical, cosmetics and food formulations.
Subject: 3-D model
cambialistic enzyme
circular dichroism
nitroblue tetrazolium
pyrogallol autoxidation
Country: EUA
Editor: Wiley-blackwell
Citation: Letters In Applied Microbiology. Wiley-blackwell, v. 57, n. 1, n. 40, n. 46, 2013.
Rights: fechado
Identifier DOI: 10.1111/lam.12071
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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