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Type: Artigo de periódico
Title: Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice
Author: Lopes, FRP
Campos, LCD
Correa, JD
Balduino, A
Lora, S
Langone, F
Borojevic, R
Martinez, AMB
Abstract: We evaluated peripheral nerve regeneration using a tubular nerve guide of resorbable collagen filled with either bone marrow-derived cells (BMDCs) in Dulbecco's cell culture medium (DMEM) or with DMEM alone (control). The control group received just the culture medium (vehicle). The left sciatic nerves of ten isogenic mice were transected and the tubular nerve guides were sutured to the end of the proximal and distal nerve stumps. Motor function was tested at 2, 4 and 6 weeks after Surgery using the walking track test. The pawprints were analyzed and the print lengths (PL) were measured to evaluate functional recovery. After 6 weeks, mice were anesthetized, perfused transcardially with fixative containing aldehydes, and the sciatic nerves and tubes were dissected and processed for scanning and transmission electron microscopy. Scanning electron microscopy of the collagen tube revealed that the tube wall became progressively thinner after surgery, proving that the tube can be resorbed in vivo. Quantitative analysis of the regenerating nerves showed that the number of myelinated fibers and the myelin area were significantly increased in the experimental group. Also, motor function recovery was faster in animals that received the cell grafts. These results indicate that the collagen tube filled with BMDCs provided an adequate and favorable environment for the growth and myelination of regenerating axons compared to the collagen tube alone. (C) 2005 Elsevier Inc. All rights reserved.
Subject: peripheral nerve regeneration
bone marrow-derived cells
collagen tube
Schwann cells
electron microscopy
Country: EUA
Editor: Academic Press Inc Elsevier Science
Citation: Experimental Neurology. Academic Press Inc Elsevier Science, v. 198, n. 2, n. 457, n. 468, 2006.
Rights: fechado
Identifier DOI: 10.1016/j.expneurol.2005.12.019
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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