Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/77971
Type: Artigo de periódico
Title: TNF-alpha TRANSIENTLY INDUCES ENDOPLASMIC RETICULUM STRESS AND AN INCOMPLETE UNFOLDED PROTEIN RESPONSE IN THE HYPOTHALAMUS
Author: Denis, RG
Arruda, AP
Romanatto, T
Milanski, M
Coope, A
Solon, C
Razolli, DS
Velloso, LA
Abstract: In diet-induced obesity, hypothalamic inflammation is triggered as an outcome of prolonged exposure to dietary fats. Toll-like receptor 4 (TLR4) activation plays a central role in this process, inducing endoplasmic reticulum stress and activating inflammatory cytokine gene transcription. Although saturated fatty acids can induce endoplasmic reticulum stress in the hypothalamus, it is unknown whether inflammatory cytokines alone can activate this mechanism. Here, rats were treated with TNF-alpha or lyposaccharide (LPS) and endoplasmic reticulum stress and unfolded protein response were evaluated by immunoblot and polymerase chain reaction (PCR). Activation of TLR4 by LPS was capable of inducing a complete endoplasmic reticulum stress and unfolded protein response through the PERK/eIF2 alpha and IRE1 alpha/XBP1 pathways. Conversely, TNF-a, injected either locally or systemically, was unable to induce a complete program of unfolded protein response, although the activation of endoplasmic reticulum stress was achieved to a certain degree. Thus, in the hypothalamus, the isolated action of TNF-alpha is insufficient to produce the activation of a complete program of unfolded protein response. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved. Key words: obesity, diabetes, food intake, leptin, insulin, cytokine.
Subject: obesity
diabetes
food intake
leptin
insulin
cytokine
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.neuroscience.2010.08.013
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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