Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/77962
Type: Artigo de periódico
Title: TITRATION OF PROTEINS IN DIMETHYL-SULFOXIDE WATER MIXTURES
Author: GODINHO, OES
RAIMUNDO, IM
ALEIXO, LM
NETO, GO
Abstract: The acid-base behaviour of arginine, lysozyme and ovalbumin has been studied by potentiometric and catalytic thermometric titrimetry in a mixture of dimethyl sulphoxide-water, with acrylonitrile as the end-point indicator in the latter technique. It was observed that, with the exception of the SH groups, all the protonated groups, including the guanidine groups of lysozyme and ovalbumin, were titrated by catalytic thermometric titrimetry. By using potentiometric titrimetry, all the titratable groups of ovalbumin were determined, whereas the guanidine groups of lysozyme were not determined by this technique.
Subject: PROTEIN
CATALYTIC TITRIMETRY
THERMOMETRIC TITRIMETRY
POTENTIOMETRIC TITRATION
DIMETHYL SULFOXIDE
Country: Inglaterra
Editor: Royal Soc Chemistry
Rights: aberto
Identifier DOI: 10.1039/an9911601181
Date Issue: 1991
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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