Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/77887
Type: Artigo de periódico
Title: Antioxidant activity and polyphenol contents in Brazilian green propolis extracts prepared with the use of ethanol and water as solvents in different pH values
Author: Mello, BCBS
Hubinger, MD
Abstract: Propolis is a natural product with a variable and complex chemical composition associated with its high concentration on polyphenolic compounds. The effect of pH variation (2.0, 3.0, 4.3, 6.0 and 8.0) during aqueous and ethanolic propolis extraction was studied for up to 10 days. Total phenols and flavonoids contents were measured for the extracts by spectrophotometric assays. Antioxidant activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and ferric thiocyanate (FTC) methods. The best time of extraction was up to 5 days for all samples. Basic aqueous extracts (pH 8.0) resulted in a higher concentration of compounds than the extract without pH modification, reaching an increase of 160% in flavonoids and 25% in phenols. Ethanolic extracts with pH variation resulted in an extract with 50% less polyphenols and 6% less flavonoids than extract without pH modification. The antioxidant activity was highest for ethanolic extract at pH 4.3 and aqueous extract at pH 8.0 almost 90% and 45%, respectively, in DPPH method and was related to the level of polyphenols by Pearson's correlations.
Subject: Antioxidant activity
extraction
flavonoids
pH
phenolic compounds
propolis
Country: EUA
Editor: Wiley-blackwell
Rights: fechado
Identifier DOI: 10.1111/j.1365-2621.2012.03129.x
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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