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Type: Artigo de periódico
Title: Time-dependent increases in ouabain-sensitive Na+, K+-ATPase activity in aortas from diabetic rats: The role of prostanoids and protein kinase C
Author: Gallo, LC
Davel, APC
Xavier, FE
Rossoni, LV
Abstract: Aims: Na+, K+-ATPase activity contributes to the regulation of vascular contractility and it has been suggested that vascular Na+, K+-ATPase activity may be altered during the progression of diabetes; however the mechanisms involved in the altered Na+, K+-ATPase activity changes remain unclear. Thus, the aim of the present study was to evaluate ouabain-sensitive Na+, K+-ATPase activity and the mechanism(s) responsible for any alterations on this activity in aortas from 1- and 4-week streptozotocin-pretreated (50 mg kg(-1), i.v.) rats. Main methods: Aortic rings were used to evaluate the relaxation induced by KCl (1-10 mM) in the presence and absence of ouabain (0.1 mmol/L) as an index of ouabain-sensitive Na+, K+-ATPase activity. Protein expression of COX-2 and p-PKC-beta II in aortas were also investigated. Key findings: Ouabain-sensitive Na+, K+-ATPase activity was unaltered following 1-week of streptozotocin administration, but was increased in the 4-week diabetic aorta (27%). Endothelium removal or nitric oxide synthase inhibition with L-NAME decreased ouabain-sensitive Na+, K+-ATPase activity only in control aortas. In denuded aortic rings, indomethacin. NS-398, ridogrel or Go-6976 normalized ouabain-sensitive Na+, K+-ATPase activity in 4-week diabetic rats. In addition, COX-2 (51%) and p-PKC-beta II (59%) protein expression were increased in 4-week diabetic aortas compared to controls. Significance: In conclusion, diabetes led to a time-dependent increase in ouabain-sensitive Na+, K+-ATPase activity. The main mechanism involved in this activation is the release of TxA(2)/PGH(2) by COX-2 in smooth muscle cells, linked to activation of the PKC pathway. (C) 2010 Elsevier Inc. All rights reserved.
Subject: Diabetes
Ouabain-sensitive Na+, K+-ATPase activity
Protein kinase C
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Life Sciences. Pergamon-elsevier Science Ltd, v. 87, n. 41921, n. 302, n. 308, 2010.
Rights: fechado
Identifier DOI: 10.1016/j.lfs.2010.07.005
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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