Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/77812
Type: Artigo de periódico
Title: Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom
Author: Torres, AFC
Dantas, RT
Menezes, RRPPB
Toyama, MH
Filho, ED
Oliveira, MF
Nogueira, NAP
Oliveira, MR
Monteiro, HSA
Martins, AMC
Abstract: Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW (R) (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 mu g/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC(50) values of 1.94 mu g/mL and 5.49 mu g/mL. Furthermore, it repressed T. cruzi growth with an IC(50) of 1.14 mu g/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi.
Subject: L-amino oxidase
Bothrops leucurus
antimicrobial activity
Country: Brasil
Editor: Cevap-unesp
Rights: aberto
Identifier DOI: 10.1590/S1678-91992010000400012
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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