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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleTooth Bleaching Increases Dentinal Protease Activitypt_BR
dc.contributor.authorSato, Cpt_BR
dc.contributor.authorRodrigues, FApt_BR
dc.contributor.authorGarcia, DMpt_BR
dc.contributor.authorVidal, CMPpt_BR
dc.contributor.authorPashley, DHpt_BR
dc.contributor.authorTjaderhane, Lpt_BR
dc.contributor.authorCarrilho, MRpt_BR
dc.contributor.authorNascimento, FDpt_BR
dc.contributor.authorTersariol, ILSpt_BR
unicamp.authorSato, C. Rodrigues, F. A. Garcia, D. M. Tersariol, I. L. S. Univ Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, Mogi das Cruzes, Brazilpt_BR
unicamp.authorVidal, C. M. P. Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, Brazilpt_BR
unicamp.authorPashley, D. H. Georgia Hlth Sci Univ, Coll Dent Med, Dept Oral Biol, Augusta, GA USApt_BR
unicamp.authorTjaederhane, L. Univ Oulu, Inst Dent, Oulu, Finlandpt_BR
unicamp.authorCarrilho, M. R. Univ Western Ontario, London, ON, Canadapt_BR
unicamp.authorNascimento, F. D. Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, Brazilpt_BR
unicamp.authorTersariol, I. L. S. Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo, Brazilpt_BR
dc.subjecttooth bleachingpt_BR
dc.subjectcysteine proteasespt_BR
dc.subjectmatrix metalloproteinasept_BR
dc.subjectconfocal microscopypt_BR
dc.subject.wosCysteine Cathepsinspt_BR
dc.description.abstractHydrogen peroxide is an oxidative agent commonly used for dental bleaching procedures. The structural and biochemical responses of enamel, dentin, and pulp tissues to the in vivo bleaching of human (n = 20) premolars were investigated in this study. Atomic force microscopy (AFM) was used to observe enamel nanostructure. The chemical composition of enamel and dentin was analyzed by infrared spectroscopy (FTIR). The enzymatic activities of dental cathepsin B and matrix metalloproteinases (MMPs) were monitored with fluorogenic substrates. The amount of collagen in dentin was measured by emission of collagen autofluorescence with confocal fluorescence microscopy. The presence of Reactive Oxygen Species (ROS) in the pulp was evaluated with a fluorogenic 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) probe. Vital bleaching of teeth significantly altered all tested parameters: AFM images revealed a corrosion of surface enamel nanostructure; FTIR analysis showed a loss of carbonate and proteins from enamel and dentin, along with an increase in the proteolytic activity of cathepsin-B and MMPs; and there was a reduction in the autofluorescence of collagen and an increase in both cathepsin-B activity and ROS in pulp tissues. Together, these results indicate that 35% hydrogen peroxide used in clinical bleaching protocols dramatically alters the structural and biochemical properties of dental hard and soft pulp
dc.relation.ispartofJournal Of Dental Researchpt_BR
dc.relation.ispartofabbreviationJ. Dent. Res.pt_BR
dc.publisher.cityThousand Oakspt_BR
dc.publisherSage Publications Incpt_BR
dc.identifier.citationJournal Of Dental Research. Sage Publications Inc, v. 92, n. 2, n. 187, n. 192, 2013.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipNIDCR [DE 015306]pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberNIDCR [DE 015306]pt
dc.description.provenanceMade available in DSpace on 2014-08-01T18:21:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:07:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
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