Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/775
Type: Artigo de periódico
Title: Substantivity of chlorhexidine to human dentin
Author: CARRILHO, Marcela R.
CARVALHO, Ricardo M.
SOUSA, Ethan N.
NICOLAU, Jose
BRESCHI, Lorenzo
MAZZONI, Annalisa
TJADERHANE, Leo
TAY, Franklin R.
AGEE, Kelli
PASHLEY, David H.
Abstract: Objectives. To better comprehend the role of CHX in the preservation of resin-dentin bonds, this study investigated the substantivity of CHX to human dentin. Material and methods. Dentin disks (n = 45) were obtained from the mid-coronal portion of human third molars. One-third of dentin disks were kept mineralized (MD), while the other two-thirds had one of the surfaces partially demineralized with 37% phosphoric acid for 15 s (PDD) or they were totally demineralized with 10% phosphoric acid (TDD). Disks of hydroxyapatite (HA) were also prepared. Specimens were treated with: (1) 10 mu L of distilled water (controls), (2) 10 mu L of 0.2% chlorhexidine diacetate (0.2% CHX) or (3) 10 mu L of 2% chlorhexidine diacetate (2% CHX). Then, they were incubated in 1 mL of PBS (pH 7.4, 37 degrees C). Substantivity was evaluated as a function of the CHX-applied dose after: 0.5 h, 1 h, 3 h, 6 h, 24 h, 168 h (1 week), 672 h (4 weeks) and 1344 h (8 weeks) of incubation. CHX concentration in eluates was spectrophotometrically analyzed at 260 nm. Results. Significant amounts of CHX remained retained in dentin substrates (MD, PPD or TDD), independent on the CHX-applied dose or time of incubation (p < 0.05). High amounts of retained CHX onto HA were observed only for specimens treated with the highest concentration of CHX (2%) (p < 0.05). Conclusion. The outstanding substantivity of CHX to dentin and its reported effect on the inhibition of dentinal proteases may explain why CHX can prolong the durability of resin-dentin bonds. (C) 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
Subject: Dentin
Chlorhexidine
Binding
Substantivity
Country: Inglaterra
Editor: ELSEVIER SCI LTD
Citation: DENTAL MATERIALS, v.26, n.8, p.779-785, 2010
Rights: fechado
Identifier DOI: 10.1016/j.dental.2010.04.002
Address: http://dx.doi.org/10.1016/j.dental.2010.04.002
http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000279068500008&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
Date Issue: 2010
Appears in Collections:FOP - Artigos e Outros Documentos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.