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Type: Artigo de periódico
Title: Toxoplasma gondii: Genotyping patients with cerebral toxoplasmosis of strains from Brazilian AIDS by multilocus PCR-RFLP markers
Author: Ferreira, IMR
Vidal, JE
Costa-Silva, TA
Meira, CS
Hiramoto, RM
de Oliveira, ACP
Pereira-Chioccola, VL
Abstract: This study investigated the genetic characteristics of the Toxoplasma gondii strains isolated from 87 patients with cerebral toxoplasmosis and AIDS, treated in Sao Paulo State, Brazil. The laboratorial diagnosis of cerebral toxoplasmosis was based on positive serological exams and PCR of blood and/or cerebrospinal fluid. Four markers (5'-SAG2, 3'-SAG2, SAG3 and GRA6) were chosen to analyze the samples. Each having clear resolution to distinguish the three clonal lineages after PCR amplified targets were treated with restriction enzyme digestion (PCR-RFLP). The genotyping provided the following results: 40 patients (46%) were infected with strains classified as type I; 4 (4%), as type III; 13 (15%) were infected with polymorphic strains (unusual genotype); 6 patients with type I or II alleles; and 15 (17%) patients had strains not classified for any marker. PCR-RFLP, also classified 9 (11%) clinical isolates as type II, which is uncommon in South America. However, the sequencing of the nested-PCR products (of SAG3 marker) of type II and polymorphic isolates (of 5'-SAG2, SAW and GRA6 markers) showed a nucleotide polymorphism compared with the archetypal clonal genotypes (types I, II and III) and these isolates were considered as polymorphic strains. The markers used here were inappropriate to distinguish the most isolates considered as polymorphic strains. These data confirm other studies showing the high rate of genetic polymorphism in T gondii strains isolated in Brazil. (C) 2007 Elsevier Inc. All rights reserved.
Subject: Toxoplasma gondii genotyping
Brazilian patients
cerebral toxoplasmosis
Country: EUA
Editor: Academic Press Inc Elsevier Science
Citation: Experimental Parasitology. Academic Press Inc Elsevier Science, v. 118, n. 2, n. 221, n. 227, 2008.
Rights: fechado
Identifier DOI: 10.1016/j.exppara.2007.08.006
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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