Please use this identifier to cite or link to this item:
http://repositorio.unicamp.br/jspui/handle/REPOSIP/77043
Type: | Artigo de periódico |
Title: | Trypsin inhibitor from Dimorphandra mollis seeds: purification and properties |
Author: | Macedo, MLR de Matos, DGG Machado, OLT Marangoni, S Novello, JC |
Abstract: | A trypsin inhibitor from Dimorphandra mollis seeds was isolated to apparent homogeneity by a combination of ammonium sulfate precipitation, gel filtration, ion-exchange and affinity chromatographic techniques. SDS-PAGE analysis gave an apparent molecular weight of 20 kDa, and isoelectric focusing analysis demonstrated the presence of three isoforms. The partial N-terminal amino acid sequence of the purified protein showed a high degree of homology with various members of the Kunitz family of inhibitors. This inhibitor, which inhibited trypsin activity with a K-i of 5.3 x 10(-10) M, is formed by a single polypeptide chain with an arginine residue in the reactive site. (C) 2000 Elsevier Science Ltd. All rights reserved. |
Subject: | Dimorphandra mollis leguminosae trypsin inhibitor Kunitz inhibitor N-terminal sequence |
Country: | Inglaterra |
Editor: | Pergamon-elsevier Science Ltd |
Citation: | Phytochemistry. Pergamon-elsevier Science Ltd, v. 54, n. 6, n. 553, n. 558, 2000. |
Rights: | fechado |
Identifier DOI: | 10.1016/S0031-9422(00)00155-2 |
Date Issue: | 2000 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
WOS000088715000001.pdf | 159.72 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.