Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76902
Type: Artigo de periódico
Title: Adsorption of human serum proteins onto TREN-agarose: Purification of human IgG by negative chromatography
Author: Bresolin, ITL
Borsoi-Ribeiro, M
Caro, JR
dos Santos, FP
de Castro, MP
Bueno, SMA
Abstract: Tris(2-aminoethyl)amine (TREN) - a chelating agent used in IMAC - immobilized onto agarose gel was evaluated for the purification of IgG from human serum by negative chromatography. A one-step purification process allowed the recovery of 73.3% of the loaded IgG in the nonretained fractions with purity of 90-95% (based on total protein concentration and nephelometric analysis of albumin, transferrin, and immunoglobulins A, G, and M). The binding capacity was relatively high (66.63 mg of human serum protein/mL). These results suggest that this negative chromatography is a potential technique for purification of IgG from human serum. (C) 2008 Elsevier B.V. All rights reserved.
Subject: Human IgG
Purification
Negative chromatography
Human serum
TREN-agarose gel
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.jchromb.2008.11.008
Date Issue: 2009
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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