Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76900
Type: Artigo de periódico
Title: Adsorption of human immunoglobulin G onto ethacrylate and histidine-linked methacrylate
Author: Aquino, LCL
Miranda, EA
Duarte, IS
Rosa, PTV
Bueno, SMA
Abstract: The adsorption of human IgG onto GMA (a semirigid metbacrylate-based chromatography matrix) and His-GMA adsorbents was studied by chromatography and batch equilibrium binding analysis. IgG molecules adsorbed onto GMA gel by nonspecific hydrophobic interactions and the specificities were similar for both adsorbents. Adsorption data were analyzed using three isotherm models, namely the Langmuir, Freundlich and Langmuir-Freundlich models, and the adsorption parameters were computed. The experimental isotherms were best described by a combined Langmuir-Freundlich model, which indicated the presence of unequal binding sites on both adsorbents and/or positive cooperativity in the binding of the IgG molecules.
Subject: IgG
histidine
methacrylate
protein adsorption
Country: Brasil
Editor: Brazilian Soc Chemical Eng
Rights: aberto
Identifier DOI: 10.1590/S0104-66322003000300005
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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