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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleAdsorption of glucagon and insulin on an immobilized metal ion affinity chromatography silica matrixpt_BR
dc.contributor.authorFarinas, CSpt_BR
dc.contributor.authorBueno, SMApt_BR
dc.contributor.authorMiranda, EApt_BR
unicamp.author.emaileverson@feq.unicamp.brpt_BR
unicamp.authorUNICAMP, LEBp, Dept Proc Biotecnol, FEQ, BR-13083970 Campinas, SP, Brazil UNICAMP, LIMBio, Dept Proc Biotecnol, FEQ, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subject.wosPurificationpt_BR
dc.subject.wosProteinspt_BR
dc.description.abstractGlucagon is a hormone that increases blood glucose concentrations and is used as a pharmaceutical product mainly in the treatment of hypoglycaemia associated with diabetes. Given both its importance and high current cost, improved purification processes are in demand. By using immobilized metal ion affinity chromatography (IMAC), we conducted tests aimed at the purification of glucagon from complex mixtures. Adsorption studies of glucagon, insulin and a mixture of the two on adsorbents of silica-IDA-Me2+ (Cu2+, Ni2+ and Zn2+) were carried out. Fixed-bed chromatographic experiments were performed and the adsorption affinity verified for all three metals tested. The most promising condition for glucagon and insulin separation was achieved by using Ni2+ as the metal ligand and desorption with a pH step gradient. Two industrial insulin-processing fractions (one glucagon-rich and the other insulin-rich) were evaluated qualitatively under these conditions, resulting in an increase in glucagon purity for both fractions with a purification factor of five for the latter.pt
dc.description.noteO TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE FEVEREIRO DE 2015.pt
dc.relation.ispartofAdsorption Science & Technologypt_BR
dc.relation.ispartofabbreviationAdsorpt. Sci. Technol.pt_BR
dc.publisher.cityBrentwoodpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherMulti Science Publ Co Ltdpt_BR
dc.date.issued2003pt_BR
dc.identifier.citationAdsorption Science & Technology. Multi Science Publ Co Ltd, v. 21, n. 10, n. 883, n. 896, 2003.pt_BR
dc.language.isoenpt_BR
dc.description.volume21pt_BR
dc.description.issuenumber10pt_BR
dc.description.firstpage883pt_BR
dc.description.lastpage896pt_BR
dc.rightsembargopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0263-6174pt_BR
dc.identifier.wosidWOS:000220066800002pt_BR
dc.identifier.doi10.1260/02636170360744335pt_BR
dc.date.available2014-08-01T18:18:27Z
dc.date.available2015-11-26T17:59:47Z-
dc.date.accessioned2014-08-01T18:18:27Z
dc.date.accessioned2015-11-26T17:59:47Z-
dc.description.provenanceMade available in DSpace on 2014-08-01T18:18:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2003en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:59:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2003en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76899
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76899-
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