Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76822
Type: Artigo de periódico
Title: Two-dimensional electrophoresis of Cereus peruvianus (Cactaceae) callus tissue proteins
Author: Mangolin, CA
Ottoboni, LMM
Machado, MDPS
Abstract: Two-dimensional electrophoresis of Cereus peruvianus callus tissues grown in culture media containing two different 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin combinations was used to identify minor differences in polypeptide composition of these cell clones. Altered expression during growth in the two 2,4-D and kinetin combinations was apparent for 13 polypeptides when calluses in the two media were compared. The number of proteins with differential expression (presence or absence of specific spots) was higher in callus tissues cultured in the 4.0 mg/L 2,4-D and 8.0 mg/L kinetin combination than in callus tissues cultured in the 4.0 mg/L 2,4-D and 4.0 mg/L kinetin combination. The present results show that the callus tissues maintained at 4.0 mg/L 2,4-D and 8.0 mg/L kinetin can be used as a matrix for in vitro selection programs.
Subject: two-dimensional electrophoresis
cactus
callus tissues
callus tissue proteins
Cactaceae
Country: Alemanha
Editor: Wiley-v C H Verlag Gmbh
Rights: fechado
Identifier DOI: 10.1002/(SICI)1522-2683(19990301)20:3<626
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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