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Type: Artigo de periódico
Title: Two genes control aluminum tolerance in maize: Genetic and molecular mapping analyses
Author: Sibov, ST
Gaspar, M
Silva, MJ
Ottoboni, LMM
Arruda, P
Souza, AP
Abstract: We have identified two loci linked to aluminum (Al) tolerance in the maize inbred line Cat-100-6 by means of restriction fragment length polymorphism (RFLP) and bulked segregant analysis (BSA). A segregating population F-2 was obtained from a cross between Cat-100-6 (Al tolerant) x S1587-17 (Al sensitive) parents. Subsequently two DNA bulks of individuals, displaying a contrasting Al tolerance trait were generated from F-2. From a total of 158 markers used, 30 markers were identified showing polymorphism between parents and bulks. The segregation results derived from the hybridization from these 30 markers and 56 individuals from F-2 revealed 10 markers cosegregating with the Al tolerance which were located in two linkage groups. The linkage groups were composed of 6 and 4 markers, and they were mapped on the short arm of chromosomes 6 and 10, respectively. From these observations, we deduce that two loci are involved in this trait in Cat-100-6 line. QGENE software was used to study the correlation between these two loci and the trait for aluminum tolerance. The results indicate that the locus on chromosome 10 has the stronger effect, and it is responsible for the major part of the variability of the trait.
Subject: maize
aluminum tolerance
molecular mapping
somaclonal variation
Country: Canadá
Editor: Natl Research Council Canada
Rights: fechado
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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