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Type: Artigo de periódico
Title: Virus inactivation by anilinonaphthalene sulfonate compounds and comparison with other ligands
Author: Bonafe, CFS
Glaser, M
Voss, EW
Weber, G
Silva, JL
Abstract: Bis-(8-anilinonaphthalene-1-sulfonate) (bis-ANS) causes inactivation of vesicular stomatitis virus (VSV) at micromolar concentrations while butyl-ANS and ANS are effective at concentrations one and two orders of magnitude higher, respectively. VSV fully inactivated by the combined effects of 10 mu M bis-ANS and 2.5 kbar hydrostatic pressure elicited a high titer of neutralizing antibodies. Incubation of VSV with greater than or equal to 2 M urea at atmospheric pressure caused very little virus inactivation, whereas at a pressure of 2.5 kbar, 1 M urea caused inactivation that exceeded by more than two orders of magnitude the sum of the inactivating effects produced by urea and pressure separately. Measurements of bis-ANS fluorescence showed that increasing the urea concentration reduces the pressure required to disrupt the structure. We conclude that anilinonaphthalene sulfonate compounds inactivate VSV by a mechanism similar to that produced by pressure. The most effective antiviral compound was bis-ANS which can be used for the preparation of safe viral vaccines or as an antiviral drug eventually. (C) 2000 Academic Press.
Subject: vesicular stomatitis virus
pressure inactivation
Country: EUA
Editor: Academic Press Inc
Rights: fechado
Identifier DOI: 10.1006/bbrc.2000.3402
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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