Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76530
Type: Artigo de periódico
Title: A novel approach based on antigen, antibody and immune complex detection in bronchoalveolar lavage fluid samples from rats experimentally infected with Strongyloides venezuelensis
Author: Goncalves, ALR
Ribeiro, TS
Silva, CV
Ueta, MT
Costa-Cruz, JM
Abstract: This study was performed in order to develop a novel approach based on antigen, antibody and immune complex detection by enzyme-linked immunosorbent assay (ELISA) in bronchoalveolar lavage fluid (BALF) samples. For that purpose Wistar rats immunosuppressed or not were experimentally infected with Strongyloides venezuelensis. The microtiter plates were coated with alkaline parasite extract for antibody detection and with IgG anti-S. venezuelensis for antigen and immune complex detection. The immune serum was able to detect 1.56 mu g/mL of L3 antigens in BALF samples. ELISA sensitivity was 96.6%, 71.6% and 91.6% for antigen, antibody and immune complex, respectively, and the specificity was 100% for all methods. Antigen detection in BALF samples showed to be a good approach for evaluating the kinetics of infection in non immunosuppressed or immunosuppressed rats. IgG was detected in non immunosuppressed rats from day 8 p.i. and in immunosuppressed rats from day 2 p.i. Moreover, immune complex was detected during the entire kinetic for both groups. In conclusion, association of antigen, antibody and immune complex detection in BALF samples seems to be an alternative approach for early strongyloidiasis diagnosis particularly in immunosuppressed individuals. (C) 2012 Elsevier B.V. All rights reserved.
Subject: Strongyloides venezuelensis
Rats
Immunosuppression
Diagnosis
Immune complex
BALF
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.actatropica.2012.07.010
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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