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Type: Artigo de periódico
Title: Urea Amperometric Biosensors Based on Nanostructured Polypyrrole
Author: Massafera, MP
de Torresi, SIC
Abstract: In this work, we report the development of urea biosensors using macroporous polypyrrole as electrochemical transducer. Nanostructured polypyrrole was synthesized using monodispersed polystyrene spheres (460 nm) as template. Nanostructured urea biosensors were assembled after immobilization of urease (an enzyme that hydrolyses urea to ammonia) on macroporous polypyrrole, which was carried out using two different procedures: covalent attachment or layer-by-layer (lbl) deposition. Results show that lbl assembly of urease on macroporous polypyrrole gives rise to biosensors with improved detection kinetics (as demonstrated by higher I(max) values) and tunable sensitivities. Comparison with bulk urea biosensors previously described in the literature demonstrates that the use of nanostructured polypyrrole as sensing platform enhances biosensor's analytical performances, mainly due to the availability of more active sites for enzyme immobilization.
Subject: Urea
Template nanostructuration
Country: EUA
Editor: Wiley-blackwell
Rights: fechado
Identifier DOI: 10.1002/elan.201100239
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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