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Type: Artigo de periódico
Title: Ultra-high pressure homogenization treatment combined with lysozyme for controlling Lactobacillus brevis contamination in model system
Author: Tribst, AAL
Franchi, MA
Cristianini, M
Abstract: It was studied the reduction of a population of Lactobacillus brevis in phosphate buffer by the combination of ultra-high pressure homogenization (UHPH) treatment and the use of lysozyme as antimicrobial. The minimum inhibitory concentration (MIC) of lysozyme against L. brevis was 50 mg.L-1. The observed inhibition was transitory and the growth curve showed that lysozyme addition was able to delay the L. brevis growth for 2 days. Lysozyme added at MIC concentration to a suspension of L. brevis caused a reduction of 1 logarithmic cycle after two hours of contact. The UHPH treatment against L. brevis resulted in a reduction of 7 log cycles at 200 MPa. Lysozyme was resistant to UHPH (200 MPa), without loss of muramidase activity or significant loss of antimicrobial power. A combined treatment was applied using 50 mg.L-1 of lysozyme and pressure between 150 and 170 MPa; the combined effect showed a reduction of about 6 logarithmic cycles and the unaltered count of L.brevis after pressure treatment for a week, with the samples stored at room temperature (25 degrees C). (C) 2007 Elsevier Ltd. All rights reserved.
Subject: lysozyme
ultra-high pressure homogenisation (UHPH)
hurdle technology
Lactobacillus brevis
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/j.ifset.2007.07.012
Date Issue: 2008
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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