Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76323
Type: Artigo de periódico
Title: A new PCR primer for the identification of Paracoccidioides brasiliensis based on rRNA sequences coding the internal transcribed spacers (ITS) and 5.8S regions
Author: Imai, T
Sano, A
Mikami, Y
Watanabe, K
Aoki, FH
Branchini, MLM
Negroni, R
Nishimura, K
Miyaji, M
Abstract: Internal transcribed spacer (ITS) genes including the 5.8S ribosomal (r)RNA of Paracoccidioides brasiliensis were amplified and the DNA sequences were determined. Based on a comparison of the sequence information, a new polymerase chain reaction (PCR) primer pair was designed for specific amplification of DNA for P. brasiliensis. This primer pair amplified a 418-bp DNA sequence and was 100% successful in identifying 29 strains of P, brasiliensis (including the reference strains) isolated from the regions of Brazil, Costa Rica, Japan, Argentina or from different sources. The results of specificity tests of these primers to compare the fungus with those of Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Histoplasma capsulatum and Penicillium marneffei are also reported.
Subject: ITS region
Paracoccidioides brasiliensis
PCR
Country: Inglaterra
Editor: B I O S Scientific Publishers Ltd
Rights: fechado
Identifier DOI: 10.1080/714030951
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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