Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76195
Type: Artigo de periódico
Title: Vitamin D Represses Dentin Matrix Protein 1 in Cementoblasts and Osteocytes
Author: Nociti, FH
Foster, BL
Tran, AB
Dunn, D
Presland, RB
Wang, L
Bhattacharyya, N
Collins, MT
Somerman, MJ
Abstract: Calcium and phosphorus homeostasis is achieved by interplay among hormones, including 1,25(OH)(2)D-3 (1,25D), parathyroid hormone, and fibroblast growth factor 23 (FGF23), and their interactions with other proteins. For example, mutations in dentin matrix protein 1 (DMP-1) result in increased FGF23 and hypophosphatemic rickets. 1,25D is reported to modulate FGF23; thus, we hypothesized that 1,25D may be involved in modulating DMP-1 in an intermediary step. Murine cementoblasts (OCCM-30) and osteocyte-like cells (MLO-Y4 and MLO-A5), known to express DMP-1, were used to analyze effects of 1,25D on DMP-1 expression in vitro. DMP-1 mRNA levels decreased by 50% (p < .05) in the presence of 1,25D in all cell types, while use of a vitamin D receptor (VDR) agonist (EB1089) and antagonist (23S,25S)-DLAM-2P confirmed that VDR pathway activation was required for this response. Further analysis showed that histone deacetylase recruitment was necessary, but neither protein kinase A nor C pathways were required. In conclusion, our results support the hypothesis that 1,25D regulates DMP-1 expression through a VDR-dependent mechanism, possibly contributing to local changes in bone/tooth mineral homeostasis.
Subject: Vitamin D receptor
phosphate homeostasis
osteogenesis
SIBLING family
fibroblast growth factor 23
phosphate regulating endopeptidase homolog
Country: EUA
Editor: Sage Publications Inc
Rights: fechado
Identifier DOI: 10.1177/0022034513516344
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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