Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76137
Type: Artigo de periódico
Title: A new amperometric biosensor for fructose using a carbon paste electrode modified with silica gel coated with Meldola's Blue and fructose 5-dehydrogenase
Author: Garcia, CAB
Neto, GD
Kubota, LT
Grandin, LA
Abstract: A carbon paste modified with silica gel coated with Meldola's Blue and fructose 5-dehydrogenase was used to construct a new biosensor for fructose. An efficient electron transfer mediation from the reduced enzyme to the carbon paste electrode was verified, at a low applied potential of 20mV vs. SCE, where interfering reactions are minimized. A linear response range for 0.1 to 0.8 mmol(-1) fructose was adjusted by the equation I=0.0150(+/-0.006)+0.618(+/-0.011) [fructose], with a correlation coefficient of 0.999 and an rsd of 0.68% for n=7. The biosensor was used to determine fructose in sweets and fruit jellies and the results present good recovery, higher than 96% in both cases. A stable response is observed over two months, when stored in a refrigerator, or for 300 measurements in continuous use.
Subject: biosensors
fructose
Meldola's Blue mediator
modified silica gel
fructose 5-dehydrogenase
Country: Suíça
Editor: Elsevier Science Sa Lausanne
Rights: fechado
Identifier DOI: 10.1016/S0022-0728(96)04775-4
Date Issue: 1996
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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