Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76088
Type: Artigo de periódico
Title: A Modified Sample Preparation for Acrylamide Determination in Cocoa and Coffee Products
Author: Arisseto, AP
Toledo, MCD
Govaert, Y
van Loco, J
Fraselle, S
Degroodt, JM
Abstract: A modified sample preparation for acrylamide determination in difficult matrices such as cocoa and coffee by a liquid chromatography-tandem mass spectrometry method is presented in this work. For the sample pretreatment, different solvents, purification steps, and solid-phase extraction cartridges were evaluated. The analyses were carried out on a mu-Bondapak C(18) column using acrylamide-D(3) as internal standard. For identification and confirmation of acrylamide, relative retention time and two diagnostic ions were monitored. Good performance of the method was achieved by introducing a protein precipitation step with Carrez I and II solutions and a liquid-liquid extraction with ethyl acetate in combination with solid-phase extraction during the sample clean-up. A limit of detection of 10 mu g/kg, a limit of quantitation of 20 mu g/kg, mean recoveries ranging from 93 to 99%, coefficients of variation of 3.4% for repeatability, and from 1.7 to 10.8% for within-laboratory reproducibility were obtained during a laboratory validation procedure.
Subject: Acrylamide
Cocoa
Complex Matrices
LC-MS/MS
Country: EUA
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s12161-007-9001-4
Date Issue: 2008
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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