Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/76086
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleA modified carbon paste electrode with silica gel coated with Meldola's blue and salicylate hydroxylase as a biosensor for salicylatept_BR
dc.contributor.authorKubota, LTpt_BR
dc.contributor.authorMilagres, BGpt_BR
dc.contributor.authorGouvea, Fpt_BR
dc.contributor.authorNeto, GDpt_BR
dc.subjectCarbon paste electrodept_BR
dc.subjectmodified silica gelpt_BR
dc.subjectsalicylate determinationpt_BR
dc.subjectbiosensorpt_BR
dc.subjectMeldola's bluept_BR
dc.subjectsalicylate hydroxylasept_BR
dc.subject.wosPharmaceutical Formulationspt_BR
dc.subject.wosLiquid-chromatographypt_BR
dc.subject.wosEnzyme Electrodept_BR
dc.subject.wosGlucose-oxidasept_BR
dc.subject.wosAcidpt_BR
dc.subject.wosSurfacept_BR
dc.subject.wosSerumpt_BR
dc.subject.wosBloodpt_BR
dc.description.abstractA biosensor for salicylate was developed using a modified carbon paste electrode with silica gel coated with Meldola's Blue and salicylate hydroxylase. This biosensor detects the catechol produced in the enzymatic reaction through its electroxidation on the mediator. The influence of NADH/substrate ratio was verified and the biosensor presented response when this ratio was higher than 1.8. The pH of the medium significantly influences the results, requiring control within a narrow range of pH. The effect of the applied potential in the biosensor response showed that 200 mV vs SCE is the best potential to use in the measurements. The biosensor has a good linear response range of 10 to 60 mu mol L(-1), adjusted by the equation I = 11.7 (+/-1.3) + 6.78 (+/-0.03)[salicylate], with high sensitivity. The relative standard deviation for 6 measurements was 3.7%. The biosensor showed good stability for 20 days when used daily or for one month when stored in a refrigerator.pt
dc.relation.ispartofAnalytical Letterspt_BR
dc.relation.ispartofabbreviationAnal. Lett.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisherMarcel Dekker Incpt_BR
dc.date.issued1996pt_BR
dc.identifier.citationAnalytical Letters. Marcel Dekker Inc, v. 29, n. 6, n. 893, n. 910, 1996.pt_BR
dc.language.isoenpt_BR
dc.description.volume29pt_BR
dc.description.issuenumber6pt_BR
dc.description.firstpage893pt_BR
dc.description.lastpage910pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0003-2719pt_BR
dc.identifier.wosidWOS:A1996UJ04100004pt_BR
dc.date.available2014-08-01T18:14:52Z
dc.date.available2015-11-26T16:58:36Z-
dc.date.accessioned2014-08-01T18:14:52Z
dc.date.accessioned2015-11-26T16:58:36Z-
dc.description.provenanceMade available in DSpace on 2014-08-01T18:14:52Z (GMT). No. of bitstreams: 0 Previous issue date: 1996en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:58:36Z (GMT). No. of bitstreams: 0 Previous issue date: 1996en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76086
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76086-
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.