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Type: Artigo de periódico
Title: A method to estimate mitochondrial Ca2+ uptake in intact cardiac myocytes
Author: Bassani, JWM
Bassani, RA
Bers, DM
Abstract: In the present paper we describe a method to estimate mitochondrial Ca2+ uptake during the declining phase of Ca2+ transients (cell relaxation) in intact isolated myocardial cells. This method is based on inhibition of sarcoplasmic reticulum (SR) Ca2+ accumulation by caffeine, blockade of Ca2+ transport via sarcolemmal Ca2+-ATPase by treatment with carboxyeosin and inhibition of sarcolemmal Na+/Ca2+ exchange by removal of extracellular Na+ and Ca2+. Ca2+ transients were evoked in rabbit ventricular myocytes by quick and sustained caffeine application (10 mM) after a 5-min period of electrical stimulation to load the SR with Ca2+. Mitochondrial Ca2+ transport was estimated using a model described by Sipido and Wier (Journal of Physiology (1991), 435:605-630), which was originally proposed to describe Ca2+ fluxes during excitation-contraction coupling in cardiac cells. Our results indicate that, in intact rabbit myocytes, the Ca2+ flux due to net mitochondrial Ca2+ uptake may attain a value close to 1 mu M/sec.
Subject: cardiac myocytes
mitochondrial Ca2+
sarcoplasmic reticulum
Na+/Ca2+ exchange
Cytosolic Ca2+
Country: Brasil
Editor: Assoc Bras Divulg Cientifica
Citation: Brazilian Journal Of Medical And Biological Research. Assoc Bras Divulg Cientifica, v. 29, n. 12, n. 1699, n. 1707, 1996.
Rights: aberto
Date Issue: 1996
Appears in Collections:Unicamp - Artigos e Outros Documentos

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