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Type: Artigo de periódico
Title: Separation of fructooligosaccharides using zeolite fixed bed columns
Author: Kuhn, RC
Maugeri, F
Abstract: Recent studies have shown that the chromatographic separation of mixtures of monosaccharides and disaccharides may be improved by employing Y zeolites, a procedure which holds promise in the separation of oligosaccharides. In the present study, a column packed with zeolite was employed to study the separation of fructooligosaccharides (FOS). FOS were produced by an enzyme isolated from Rhodotorula sp., which produces GF(2) (kestose), GF(3) (nystose) and GF(4) (frutofuranosyl nystose). The identification and quantification of the sugars were carried out by ion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The separation of fructooligosaccharides was carried out using a fixed bed column packed with Ba(2+)-exchange Y zeolites. The effects of temperature (40-50 degrees C), injected volume per bed volume (2.55-7.64%), superficial velocity (0.1-0.15 cm min(-1)) and eluent composition (40-60% ethanol) were investigated using a fractionary factorial design with separation efficiency as the response. The results showed that the most favorable conditions for the separation of the oligosaccharide-glucose mixture were 60% ethanol as eluent, temperature of 50 degrees C, superficial velocity of 0.1 cm min(-1) and 2.55% injection volume per bed volume of injection mixture, using two columns in series. The values for separation efficiency were 0.60 for oligosaccharide-glucose, 1.00 for oligosaccharide-fructose, 0.22 for oligosaccharide-sucrose, 0.43 for glucose-fructose, 0.82 for glucose-sucrose and 1.23 for fructose-sucrose. (C) 2010 Elsevier B.V. All rights reserved.
Subject: Fixed bed column
Experimental design
Country: Holanda
Editor: Elsevier Science Bv
Citation: Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 878, n. 22, n. 2023, n. 2028, 2010.
Rights: fechado
Identifier DOI: 10.1016/j.jchromb.2010.05.039
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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