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Type: Artigo de periódico
Title: Sildenafil (Viagra) Protective Effects on Neuroinflammation: The Role of iNOS/NO System in an Inflammatory Demyelination Model
Author: Raposo, C
Nunes, AKD
Luna, RLD
Araujo, SMD
da Cruz-Hofling, MA
Peixoto, CA
Abstract: We recently demonstrated that sildenafil reduces the expression of cytokines, COX-2, and GFAP in a demyelinating model induced in wild-type (WT) mice. Herein, the understandings of the neuroprotective effect of sildenafil and the mediation of iNOS/NO system on inflammatory demyelination induced by cuprizone were investigated. The cerebella of iNOS(-/-) mice were examined after four weeks of treatment with cuprizone alone or combined with sildenafil. Cuprizone increased GFAP, Iba-1, TNF-alpha, COX-2, IL-1 beta, and IFN-gamma expression, decreased expression of glutathione S-transferase pi (GSTpi), and damaged myelin in iNOS(-/-) mice. Sildenafil reduced Iba-1, IFN-gamma, and IL-1 beta levels but had no effect on the expression of GFAP, TNF-alpha, and COX-2 compared to the cuprizone group. Sildenafil elevated GSTpi levels and improved the myelin structure/ultrastructure. iNOS(-/-) mice suffered from severe inflammation following treatment with cuprizone, while WT mice had milder inflammation, as found in the previous study. It is possible that inflammatory regulation through iNOS-feedback is absent in iNOS(-/-) mice, making them more susceptible to inflammation. Sildenafil has at least a partial anti-inflammatory effect through iNOS inhibition, as its effect on iNOS(-/-) mice was limited. Further studies are required to explain the underlying mechanism of the sildenafil effects.
Country: EUA
Editor: Hindawi Publishing Corporation
Citation: Mediators Of Inflammation. Hindawi Publishing Corporation, 2013.
Rights: aberto
Identifier DOI: 10.1155/2013/321460
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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