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Type: Artigo de periódico
Title: Short-term PTH administration increases dentine apposition and microhardness in mice
Author: Guimaraes, GN
Cardoso, GBC
Naves, LZ
Correr-Sobrinho, L
Line, SRP
Marques, MR
Abstract: Objective: The purpose of this study is to investigate the effects of intermittent parathyroid hormone (PTH) administration on the apposition rate and structural features of dentine from mouse incisors. Methods: Young male A/J Unib mice were treated daily for 6 and 10 days with 40 mu g/kg of hPTH 1-34 or a vehicle. Dentine apposition rates measured by fluorescent labels (tetracycline and calcein) and alkaline phosphatase (ALP) plasma levels were evaluated after 6 days of treatment. Knoop microhardness testing and element content measurements in at.% of calcium (Ca), phosphorus (P), oxygen (0), and magnesium (Mg) in the peritubular and intertubular dentine were performed by Energy Dispersive X-ray (EDX) microanalysis via Scanning Electron Microscopy (SEM) after 10 days of treatment. Results: Histometric analysis revealed an increase of 5% in the apposition rate of dentine and 25% in the ALP plasma levels in the PTH treated group. In addition, knoop microhardness testing revealed that the animals treated with PTH had a greater microhardness (11%). EDX microanalysis showed that PTH treatment led to increases in P (23%) and Ca (53%) at.% content, as well as the Ca/P ratio (24%) in peritubular dentine. The chemical composition of intertubular dentine did not vary between the groups. Conclusions: These findings indicate that intermittent administration of hPTH (1-34) increases apposition and mineralization of the dentine during young mice incisor formation. (c) 2012 Elsevier Ltd. All rights reserved.
Subject: Parathyroid hormone
Knoop microhardness
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.archoralbio.2012.03.007
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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