Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/752
Type: Artigo de periódico
Title: Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
Author: GONCALVES, Thais Oliveira
COSTA, Dirceu
BRODSKYN, Claudia Ida
DUARTE, Poliana Mendes
CESAR NETO, Joao Batista
NOGUEIRA-FILHO, Getulio
Abstract: Objective: The emergence of periodontal medicine increased interest in defining the behaviour of peripheral blood cells in periodontitis subjects in comparison with healthy group. The aim of this study was to evaluate the levels of interleukin (IL)-8, tumour necrosis factor-alpha (TNF-alpha), IL-6 and IL-10 released by Escherichia coli lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) obtained from the peripheral blood of chronic periodontitis subjects. Design: PBMC samples were isolated from 19 systemically healthy donors, divided into generalized chronic periodontitis (n = 10) and healthy (n = 9) subjects. Cells were incubated for 24-48 h in 500 mu L wells containing RPM! 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of IL-8, TNF-alpha, IL-6 and IL-10 released using enzyme-linked immunosorbent assay (ELISA). Results: PBMC cells from periodontitis subjects released higher levels of TNF-alpha and IL-6 than those from healthy subjects (P < 0.05). Conversely, the supernatants of the stimulated PBMC cells obtained from healthy subjects presented higher amounts of IL-8 than those from periodontitis (P < 0.05). No differences were observed in the levels of IL-10 (P > 0.05) between groups. Conclusion: In conclusion, the results of the present study showed that E. coli LPS-stimulated PBMC from subjects with periodontitis present a different pattern of cytokine release when compared to PBMC from healthy subjects. This phenomenon could have implications locally, in periodontitis, as well as in systemic diseases. (C) 2010 Elsevier Ltd. All rights reserved.
Subject: Periodontitis
Cytokines
ELISA
Mononuclear cell
PBMC
Country: Inglaterra
Editor: PERGAMON-ELSEVIER SCIENCE LTD
Citation: ARCHIVES OF ORAL BIOLOGY, v.55, n.12, p.975-980, 2010
Rights: fechado
Identifier DOI: 10.1016/j.archoralbio.2010.08.002
Address: http://dx.doi.org/10.1016/j.archoralbio.2010.08.002
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Date Issue: 2010
Appears in Collections:FOP - Artigos e Materiais de Revistas Científicas

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