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Type: Artigo
Title: Suppressor of cytokine signaling-3 provides a novel interface in the cross-talk between angiotensin II and insulin signaling systems
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Author: Velloso, L.A.
Calegari, V.C.
Saad, M.J.A.
Alves, M.
Inoue, R.Y.
Picardi, P.K.
Franchini, K.G.
Abstract: Angiotensin II inhibits insulin-induced activation of phosphatidylinositol 3-kinase through a mechanism, at least in part, dependent on serine phosphorylation of the insulin receptor and insulin receptor substrates (IRS)-1/2. Recent evidence shows that suppressor of cytokine signaling-3 (SOCS-3) is induced by insulin and angiotensin II and participates in the negative control of further stimulation of each of these signaling systems independently. In the present study, we evaluated the interaction of angiotensin II-induced SOCS-3 with the insulin signaling pathway in the heart of living rats. A single iv dose of angiotensin II promotes a significant increase of SOCS-3 in heart, an effect that lasts up to 180 min. Once induced, SOCS-3 interacts with the insulin receptor, JAK-2, IRS-1, and IRS-2. The inhibition of SOCS-3 expression by a phosphorthioate-modified antisense oligonucleotide partially restores angiotensin II-induced inhibition of insulin-induced insulin receptor, IRS-1 and IRS-2 tyrosine phosphorylation, and IRS-1 and IRS-2 association with p85-phosphatidylinositol 3-kinase and [Ser(473)] phosphorylation of Akt. Moreover, the inhibition of SOCS-3 expression partially reverses angiotensin II-induced inhibition of insulin-stimulated glucose transporter-4 translocation to the cell membrane. These results are reproduced in isolated cardiomyocytes. Thus, SOCS-3 participates, as a late event, in the negative crosstalk between angiotensin II and insulin, producing an inhibitory effect on insulin-induced glucose transporter-4 translocation
Subject: Angiotensina II
Proteínas de ligação a DNA
Expressão gênica
Miócitos cardíacos
Country: Estados Unidos
Editor: Oxford University Press
Citation: Endocrinology. Endocrine Soc, v. 146, n. 2, n. 579, n. 588, 2005.
Rights: fechado
Identifier DOI: 10.1210/en.2004-0466
Date Issue: 2005
Appears in Collections:FCM - Artigos e Outros Documentos

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