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Type: Artigo de periódico
Title: Supercritical fluid extraction of Agaricus brasiliensis: Antioxidant and antimicrobial activities
Author: Mazzutti, S
Ferreira, SRS
Riehl, CAS
Smania, A
Smania, FA
Martinez, J
Abstract: The objective of this study was to obtain extracts from the mushroom Agaricus brasiliensis using supercritical fluid extraction (SFE) with pure CO2 and with CO2 plus 2.5%, 5.0% and 10.0% (w/w) of ethanol as co-solvent. In order to evaluate the high-pressure method in terms of process yield, extract composition and biological activity, low-pressure methods, such as maceration with ethanol (Mac), Soxhlet (Sox) with different organic solvents, and hydrodistillation (HD), were also applied to obtain extracts. The SFE conditions were temperatures of 313.15K, 323.15K and 333.15K and pressures from 10 to 30 MPa. The SFE kinetics was investigated through the overall extraction curve (OEC). The extracts obtained by Sox with water and ethanol showed the best results for the global extraction yield. The best conditions in the studied range to obtain high yields using pure CO2 resulted to be 30.0 MPa and 323.15K. The antioxidant potential of the extracts was evaluated by the DPPH method and by the Folin-Ciocalteau method. Maceration extract presented low yield, but good results of antioxidant activity by DPPH assays and total phenolic. The antimicrobial activity of the extracts was also studied. The main identified compounds in the extracts were linoleic and palmitic acids. (C) 2012 Elsevier B.V. All rights reserved.
Subject: Supercritical fluid extraction
Antioxidant activity
Antimicrobial activity
Agaricus brasiliensis
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.supflu.2012.06.010
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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