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|Type:||Artigo de periódico|
|Title:||Sugarcane genome sequencing and gene discovery: Getting closer to sugar content, fibre and drought traits|
|Abstract:||The understanding of the regulatory networks that drive sugarcane physiology and agronomic traits becomes increasingly necessary for the improvement of sugarcane. We are interested in understanding the regulation of carbon partitioning, sugar content, fibre yield and drought tolerance. Different field and greenhouse experiments have been done to obtain physiological and technological data. We collected sugarcane samples from leaf and internode tissues and analysed transcriptome and biochemical changes. Data are being integrated in the SUCEST-FUN database (http://sucest-fun.org) where several tools are available to interrogate the data focusing on different aspects. We have identified 10 262 differences in gene expression when cultivars and tissues with contrasting sucrose content were compared, 12 249 changes related to drought stress and 3524 when ancestral sugarcane species were compared to a commercial sugarcane cultivar with differing fibre deposition patterns. We are developing techniques to implement sugarcane regulatory network studies using a systems biology approach. We are identifying antisense transcripts related to the traits of interest, gene upstream and downstream promoters, and active promoters using Chip-seq combined with 454 sequencing. We are sequencing the sugarcane genome using the 454 platform and the Whole Genome Shotgun and BAC approaches. With the 454 WGS sequencing, we already obtained 10.8 Gb of data. Using sugarcane 454 sequences, we were able to confirm gene structure and to clone promoter sequences. The transcriptomic and genomic techniques that are being developed in our group associated with the physiological data are resulting in a more comprehensive understanding of sugar content, fibre and drought regulation.|
|Country:||País de Gales|
|Editor:||Int Sugar Journal Ltd|
|Appears in Collections:||Artigos e Materiais de Revistas Científicas - Unicamp|
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