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Type: Artigo de periódico
Title: Stability of free and immobilized Lactobacillus acidophilus and Bifidobacterium lactis in acidified milk and of immobilized B-lactis in yoghurt
Author: Grosso, CRF
Favaro-Trindade, CS
Abstract: This study evaluated the stability of Bifidobacterium lactis (Bb-12) and of Lactobacillus acidophilus (La05) both free and immobilized in calcium alginate, in milk and in acidified milk (pH 5.0,4.4 and 3.8). The stability of immobilized B. lactis in yoghurt (fermented to pH 4.2), during 28 days of refrigerated storage was also evaluated. The efficiency Of two Culture media (modified MRS agar and Reinforced Clostridial Agar plus Prussian Blue) for counting of B. lactis ill yoghurt was determined. Lee's agar was used to count Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus when B. lactis were counted in the MRS medium. B. lactis and L. acidophilus in both free and immobilized forms presented satisfactory rates of survival ill milk and acidified milk because the average reduction of the Population was only one log cycle after 21 days of storage. The number of viable cells of immobilized B. lactis in yoghurt presented a gradual decline throughout the storage period, passing from 10(8) cfu/ml to no Count after 28 days of storage. When the Cultures were not in equilibrium just the selective medium was efficient in counting B. lactis ill yoghurt. The results showed that both microorganisms call be added to milk and acidified milk, because their population was only slightly affected during storage. The presence of traditional culture of yoghurt seems to be harmful for Survival of immobilized B. lactis and the immobilization in calcium alginate failed as an effective barrier to protect the cells in all analysed treatments.
Subject: probiotics
Lactobacillus acidophilus
Bifidobacterium lactis
Lactobacillus bulgaricus
Country: Brasil
Editor: Soc Brasileira Microbiologia
Rights: aberto
Identifier DOI: 10.1590/S1517-83822004000100025
Date Issue: 2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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